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Journal of Clinical Microbiology, July 2004, p. 3336-3338, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.3336-3338.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Direct Identification of Mycobacterium haemophilum in Skin Lesions of Immunocompromised Patients by PCR-Restriction Endonuclease Analysis

S. X. Wang,1* L. H. Sng,1 H. N. Leong,2 and B. H. Tan2

Central Tuberculosis Laboratory, Department of Pathology,1 Department of Internal Medicine, Singapore General Hospital, Republic of Singapore2

Received 12 November 2003/ Returned for modification 28 January 2004/ Accepted 22 March 2004

PCR-restriction endonuclease analysis (PRA) was used for direct identification of Mycobacterium haemophilum in clinical specimens from immunocompromised patients. PRA correctly identified M. haemophilum in four smear-positive specimens. Direct identification by PRA takes 2 to 3 working days compared to the 3 to 5 weeks required for culture isolation and identification by conventional methods.

* Corresponding author. Mailing address: Central Tuberculosis Laboratory, Department of Pathology, Singapore General Hospital, Outram Rd., Singapore 169608. Phone: (65)-62221391. Fax: (65)-62226826. E-mail: gptwsx{at}sgh.com.sg.

Journal of Clinical Microbiology, July 2004, p. 3336-3338, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.3336-3338.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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