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Journal of Clinical Microbiology, July 2004, p. 3339-3341, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.3339-3341.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Diagnostic Utility and Clinical Significance of Naso- and Oropharyngeal Samples Used in a PCR Assay To Diagnose Mycoplasma pneumoniae Infection in Children with Community-Acquired Pneumonia

Ian C. Michelow,1 Kurt Olsen,1 Juanita Lozano,1 Lynn B. Duffy,2 George H. McCracken,1 and R. Doug Hardy1*

Departments of Pediatrics and Internal Medicine (Divisions of Infectious Diseases), University of Texas Southwestern Medical Center, Dallas, Texas,1 Diagnostic Mycoplasma Laboratory, University of Alabama, Birmingham, Alabama2

Received 29 December 2003/ Returned for modification 3 March 2004/ Accepted 1 April 2004

PCR assays of naso- and oropharyngeal samples among hospitalized children appear equally effective for the diagnosis of serologically confirmed community-acquired mycoplasmal pneumonia. However, the combination of results from both sites yields optimal sensitivity (57%), specificity (98%), and positive (92%) and negative (82%) predictive values when compared with Mycoplasma pneumoniae enzyme-linked immunosorbent assay.

* Corresponding author. Mailing address: Departments of Pediatrics and Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 75390-9063. Phone: (214) 648-3720. Fax: (214) 648-2961. E-mail: robert.hardy{at}utsouthwestern.edu.

Journal of Clinical Microbiology, July 2004, p. 3339-3341, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.3339-3341.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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