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Selected Pool of Peptides from ESAT-6 and CFP-10 Proteins for Detection of Mycobacterium tuberculosis Infection

Paolo Scarpellini,^1, Silvana Tasca,^1,2, Laura Galli,³ Alberto Beretta,¹ Adriano Lazzarin,^1,4 and Claudio Fortis^1,2*

Clinic of Infectious Diseases,¹ Laboratory of Clinical Immunology,² San Raffaele Hospital "Vita-Salute" San Raffaele University,³ San Raffaele Scientific Institute, Milan, Italy⁴

Received 11 December 2003/ Returned for modification 27 February 2004/ Accepted 12 May 2004

We have validated a new test for detecting Mycobacterium tuberculosis infection. A pool of synthetic peptides derived from ESAT-6 and CFP-10 proteins was used to detect the number of specific gamma interferon-producing T cells by means of an enzyme-linked immunospot assay. Sixty-eight individuals positive for M. tuberculosis infection, either human immunodeficiency virus-seropositive or -seronegative, were studied. The test results were highly specific (87.5%) and sensitive (93.1%), more so than a classical lymphoproliferative assay (specificity and sensitivity of 77.27%), opening new possibilities for diagnosis and screening of tuberculosis. Moreover, the test allowed us to distinguish individuals infected with M. tuberculosis from those vaccinated with BCG.

P.S. and S.T. contributed equally to this study.

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