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Journal of Clinical Microbiology, August 2004, p. 3518-3524, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3518-3524.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Alanine-Threonine Polymorphism of Helicobacter pylori RpoB Is Correlated with Differential Induction of Interleukin-8 in MKN45 Cells

Keun-Hwa Lee,1,{dagger} Myung-Je Cho,2 Yoshio Yamaoka,3 David Y. Graham,3 Yeo-Jun Yun,1 So-Yon Woo,1 Chang-Young Lim,4 Kwan-Soo Ko,1 Bum-Joon Kim,1 Hyun-Chae Jung,5 Woo-Kon Lee,2 Kwang-Ho Rhee,2 and Yoon-Hoh Kook1*

Department of Microbiology and Cancer Research Institute, Institute of Endemic Diseases, SNUMRC, Seoul National University College of Medicine, and Clinical Research Institute, Seoul National University Hospital,1 Department of Internal Medicine, Hansol Hospital, Sukchondong, Songpagu,4 Department of Internal Medicine, Seoul National University College of Medicine, Seoul,5 Department of Microbiology, Gyeong-Sang National University College of Medicine, Chinju, South Korea,2 Department of Medicine, Veterans Affairs Medical Center, Baylor College of Medicine, Houston, Texas3

Received 9 January 2004/ Returned for modification 3 March 2004/ Accepted 6 May 2004

Geographical differences in the genetic diversity of Helicobacter pylori isolates were examined by analyzing rpoB sequences. An extremely high level of allelic diversity among H. pylori strains was found. The rpoB sequences of Asian and non-Asian (North and South American, European, and South African) strains were found to differ. An amino acid polymorphism (alanine and threonine RpoB types) was found at the 497th residue by deduced amino acid analysis. RpoB with a threonine residue (RpoBThr) was uniquely present in East Asian countries, and two-thirds of the H. pylori isolate population in this region was RpoBThr; however, this type was rare or absent in Western countries, where RpoBAla predominated. RpoBThr strains induced a much larger amount of interleukin-8, a chemokine that plays an important role in chronic inflammation, than RpoBAla strains in cultured MKN45 cells.

* Corresponding author. Mailing address: Department of Microbiology, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul, 110-799, Korea. Phone: (82) 2-740-8306. Fax: (82) 2-743-0881. E-mail: yhkook{at}plaza.snu.ac.kr.

{dagger} Present address: Department of Microbiology, Cheju National University College of Medicine, Jeju 690-756, Korea.

Journal of Clinical Microbiology, August 2004, p. 3518-3524, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3518-3524.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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