This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ariga, T. Articles by Ohno, S. Search for Related Content PubMed PubMed Citation Articles by Ariga, T. Articles by Ohno, S.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 2004, p. 3644-3648, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3644-3648.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

New Genome Type of Adenovirus Serotype 4 Caused Nosocomial Infections Associated with Epidemic Conjunctivitis in Japan

Department of Ophthalmology and Visual Sciences, Hokkaido University Graduate School of Medicine, Kita-ku, Sapporo, Hokkaido 060-8638,¹ Research and Development Department, Mitsubishi Kagaku Biochemical Laboratories Inc., Shimura 3-30-1, Itabashi-ku, Tokyo 174-8555, Japan²

Received 30 October 2003/ Returned for modification 16 December 2003/ Accepted 28 April 2004

Human adenovirus type 4 is one of the major serotypes isolated from patients with adenoviral conjunctivitis. In 2001 we encountered nosocomial infections with epidemic conjunctivitis in the ophthalmology ward of one hospital in Sapporo, which is in the northern part of Japan. Adenoviruses were isolated from the patients with this nosocomial infection and identified as adenovirus type 4 (AdV-4) by a neutralization test with serotype-specific antiserum. When the cleavage patterns of the isolates were compared with the full viral genome with BamHI and SmaI, the cleavage patterns of the isolates were shown to be different from those of AdV-4p and other previously known AdV-4 variants. The nucleotide sequences of the fiber gene of the isolates showed the highest homologies (94.3%) with AdV-4 among the nucleotide sequences available from GenBank and formed a monophyletic cluster along with the prototype strain of AdV-4. The isolates, however, were located in a different lineage from those of AdV-4p and the AdV-4 variant from the sporadic infections. We conclude that the nosocomial infection that appeared in 2001 was caused by a new genome type of AdV-4, which was designated AdV-4c.

This article has been cited by other articles:

• Ishiko, H., Shimada, Y., Konno, T., Hayashi, A., Ohguchi, T., Tagawa, Y., Aoki, K., Ohno, S., Yamazaki, S. (2008). Novel Human Adenovirus Causing Nosocomial Epidemic Keratoconjunctivitis. J. Clin. Microbiol. 46: 2002-2008 [Abstract] [Full Text]  
• Casas, I., Avellon, A., Mosquera, M., Jabado, O., Echevarria, J. E., Campos, R. H., Rewers, M., Perez-Brena, P., Lipkin, W. I., Palacios, G. (2005). Molecular Identification of Adenoviruses in Clinical Samples by Analyzing a Partial Hexon Genomic Region. J. Clin. Microbiol. 43: 6176-6182 [Abstract] [Full Text]  
• Ariga, T., Shimada, Y., Shiratori, K., Ohgami, K., Yamazaki, S., Tagawa, Y., Kikuchi, M., Miyakita, Y., Fujita, K., Ishiko, H., Aoki, K., Ohno, S. (2005). Five New Genome Types of Adenovirus Type 37 Caused Epidemic Keratoconjunctivitis in Sapporo, Japan, for More Than 10 Years. J. Clin. Microbiol. 43: 726-732 [Abstract] [Full Text]