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Journal of Clinical Microbiology, August 2004, p. 3649-3654, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3649-3654.2004

Use of 16S rRNA Gene Sequencing for Rapid Confirmatory Identification of Brucella Isolates

Jay E. Gee,* Barun K. De, Paul N. Levett,{dagger} Anne M. Whitney, Ryan T. Novak, and Tanja Popovic

Meningitis and Special Pathogens Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333

Received 2 January 2004/ Returned for modification 22 February 2004/ Accepted 24 April 2004

Members of the genus Brucella are categorized as biothreat agents and pose a hazard for both humans and animals. Current identification methods rely on biochemical tests that may require up to 7 days for results. We sequenced the 16S rRNA genes of 65 Brucella strains along with 17 related strains likely to present a differential diagnostic challenge. All Brucella 16S rRNA gene sequences were determined to be identical and were clearly different from the 17 related strains, suggesting that 16S rRNA gene sequencing is a reliable tool for rapid genus-level identification of Brucella spp. and their differentiation from closely related organisms.

* Corresponding author. Mailing address: Meningitis and Special Pathogens Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, MS-D11, 1600 Clifton Rd., N.E., Atlanta, GA 30333. Phone: (404) 639-4936. Fax: (404) 639-4421. E-mail: JGee1{at}cdc.gov.

{dagger} Present address: Saskatchewan Health, Provincial Laboratory, 3211 Albert St., Regina, Saskatchewan, Canada S4S 5W6.

Journal of Clinical Microbiology, August 2004, p. 3649-3654, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3649-3654.2004




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