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Journal of Clinical Microbiology, August 2004, p. 3661-3664, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3661-3664.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Comparison of a New Lateral-Flow Chromatographic Membrane Immunoassay to Viral Culture for Rapid Detection and Differentiation of Influenza A and B Viruses in Respiratory Specimens

Andreea C. Cazacu,¹ Gail J. Demmler,^1,2* Mark A. Neuman,³ Betty A. Forbes,⁴ Sooyoung Chung,⁵ Jewel Greer,^1,2 Ana E. Alvarez,³ Robin Williams,³ and Nadine Y. Bartholoma⁴

Department of Pediatrics, Baylor College of Medicine,¹ Diagnostic Virology Laboratory, Texas Children's Hospital, Houston, Texas,² DSI Laboratories, Division of Infectious Diseases, University of South Florida College of Medicine, Ft. Myers, Florida,³ Microbiology Laboratory, SUNY Upstate Medical University, Syracuse,⁴ New York University School of Medicine, New York, New York⁵

Received 29 February 2004/ Returned for modification 6 April 2004/ Accepted 26 April 2004

The performance of a new rapid lateral-flow chromatographic membrane immunoassay test kit for detection of influenza virus was evaluated and compared to that of viral culture in respiratory secretions collected from 400 adults and children seen at three large university hospitals during the recent 2003 influenza season. The rapid test provided results in 15 min, with excellent overall performance statistics (sensitivity, 94.4%; specificity, 100%; positive predictive value, 100%; negative predictive value, 97.5%). Both influenza A and B type viruses were reliably detected, with no significant difference in performance statistics noted by influenza virus type or by the center performing the test.

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* Corresponding author. Mailing address: Pediatric Infectious Diseases, Feigin Center MC3-2371, Texas Children's Hospital, 6621 Fannin, Houston, TX 77030. Phone: (832) 824-4330. Fax: (832) 825-4347. E-mail: gdemmler{at}bcm.tmc.edu.

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Journal of Clinical Microbiology, August 2004, p. 3661-3664, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3661-3664.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

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