This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cazacu, A. C.
Right arrow Articles by Demmler, G. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cazacu, A. C.
Right arrow Articles by Demmler, G. J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 2004, p. 3707-3710, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3707-3710.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Comparison of the Directigen Flu A+B Membrane Enzyme Immunoassay with Viral Culture for Rapid Detection of Influenza A and B Viruses in Respiratory Specimens

Andreea C. Cazacu,1 Sooyoung E. Chung,2 Jewel Greer,1,3 and Gail J. Demmler1,3*

Department of Pediatrics, Baylor College of Medicine,1 Diagnostic Virology Laboratory, Texas Children's Hospital, Houston, Texas 77030,3 New York University School of Medicine, New York, New York 100162

Received 15 December 2003/ Returned for modification 7 February 2004/ Accepted 29 March 2004

The performance of a commercially available, rapid membrane enzyme immunoassay for influenza A and B virus detection was compared to that of viral culture in 4,092 respiratory specimens collected from patients presenting with respiratory symptoms during the 2002-2003 influenza season. The test's overall sensitivity was 43.83%, lower than previously reported but similar for detection of both influenza A and B viruses (42.98 versus 44.76%). However, specificity, 99.74%, was excellent for both influenza A and B viruses (99.82 versus 99.92%). These values make this test a very good confirmatory test when clinical suspicion is high, but a less accurate screening test for large populations.

* Corresponding author. Mailing address: Pediatric Infectious Diseases, Feigin Center MC3-2371, Texas Children's Hospital, 6621 Fannin, Houston, TX 77030. Phone: (832) 824-4330. Fax: (832) 825-4347. E-mail: gdemmler{at}bcm.tmc.edu.

Journal of Clinical Microbiology, August 2004, p. 3707-3710, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3707-3710.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Liao, R. S., Tomalty, L. L., Majury, A., Zoutman, D. E. (2009). Comparison of Viral Isolation and Multiplex Real-Time Reverse Transcription-PCR for Confirmation of Respiratory Syncytial Virus and Influenza Virus Detection by Antigen Immunoassays. J. Clin. Microbiol. 47: 527-532 [Abstract] [Full Text]  
  • Dale, S. E., Mayer, C., Mayer, M. C., Menegus, M. A. (2008). Analytical and Clinical Sensitivity of the 3M Rapid Detection Influenza A+B Assay. J. Clin. Microbiol. 46: 3804-3807 [Abstract] [Full Text]  
  • Roh, K. H., Kim, J., Nam, M.-H., Yoon, S., Lee, C. K., Lee, K., Yoo, Y., Kim, M. J., Cho, Y. (2008). Comparison of the Seeplex Reverse Transcription PCR Assay with the R-mix Viral Culture and Immunofluorescence Techniques for Detection of Eight Respiratory Viruses. Annals of Clinical & Laboratory Science 38: 41-46 [Abstract] [Full Text]  
  • Leland, D. S., Ginocchio, C. C. (2007). Role of Cell Culture for Virus Detection in the Age of Technology. Clin. Microbiol. Rev. 20: 49-78 [Abstract] [Full Text]  
  • Grijalva, C. G., Poehling, K. A., Edwards, K. M., Weinberg, G. A., Staat, M. A., Iwane, M. K., Schaffner, W., Griffin, M. R. (2007). Accuracy and Interpretation of Rapid Influenza Tests in Children. Pediatrics 119: e6-e11 [Abstract] [Full Text]  
  • Agoritsas, K., Mack, K., Bonsu, B. K., Goodman, D., Salamon, D., Marcon, M. J. (2006). Evaluation of the Quidel QuickVue Test for Detection of Influenza A and B Viruses in the Pediatric Emergency Medicine Setting by Use of Three Specimen Collection Methods.. J. Clin. Microbiol. 44: 2638-2641 [Abstract] [Full Text]  
  • Fader, R. C. (2005). Comparison of the Binax NOW Flu A Enzyme Immunochromatographic Assay and R-Mix Shell Vial Culture for the 2003-2004 Influenza Season. J. Clin. Microbiol. 43: 6133-6135 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»