This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An erratum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bavykin, S. G.
Right arrow Articles by Cherni, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bavykin, S. G.
Right arrow Articles by Cherni, A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 2004, p. 3711-3730, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3711-3730.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Use of 16S rRNA, 23S rRNA, and gyrB Gene Sequence Analysis To Determine Phylogenetic Relationships of Bacillus cereus Group Microorganisms

Sergei G. Bavykin,1* Yuri P. Lysov,1,2 Vladimir Zakhariev,1,2 John J. Kelly,1,3 Joany Jackman,4 David A. Stahl,5 and Alexey Cherni1,2

BioChip Technology Center, Argonne National Laboratory, Argonne, Illinois, 60439,1 Engelhardt Institute of Molecular Biology, Moscow 117984, Russia,2 Department of Biology, Loyola University Chicago, Chicago, Illinois 60626,3 Applied Physics Laboratory, Johns Hopkins University, Laurel, Maryland 20723,4 Department of Civil and Environmental Engineering, University of Washington, Seattle, Washington 981955

Received 1 December 2003/ Returned for modification 23 February 2004/ Accepted 22 April 2004

In order to determine if variations in rRNA sequence could be used for discrimination of the members of the Bacillus cereus group, we analyzed 183 16S rRNA and 74 23S rRNA sequences for all species in the B. cereus group. We also analyzed 30 gyrB sequences for B. cereus group strains with published 16S rRNA sequences. Our findings indicated that the three most common species of the B. cereus group, B. cereus, Bacillus thuringiensis, and Bacillus mycoides, were each heterogeneous in all three gene sequences, while all analyzed strains of Bacillus anthracis were found to be homogeneous. Based on analysis of 16S and 23S rRNA sequence variations, the microorganisms within the B. cereus group were divided into seven subgroups, Anthracis, Cereus A and B, Thuringiensis A and B, and Mycoides A and B, and these seven subgroups were further organized into two distinct clusters. This classification of the B. cereus group conflicts with current taxonomic groupings, which are based on phenotypic traits. The presence of B. cereus strains in six of the seven subgroups and the presence of B. thuringiensis strains in three of the subgroups do not support the proposed unification of B. cereus and B. thuringiensis into one species. Analysis of the available phenotypic data for the strains included in this study revealed phenotypic traits that may be characteristic of several of the subgroups. Finally, our results demonstrated that rRNA and gyrB sequences may be used for discriminating B. anthracis from other microorganisms in the B. cereus group.

* Corresponding author. Mailing address: BioChip Technology Center, Argonne National Laboratory, Argonne, IL 60439. Phone: (630) 252-3980. Fax: (630) 252-9155. E-mail: sbavykin{at}anl.gov.

Journal of Clinical Microbiology, August 2004, p. 3711-3730, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3711-3730.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • McIntyre, L., Bernard, K., Beniac, D., Isaac-Renton, J. L., Naseby, D. C. (2008). Identification of Bacillus cereus Group Species Associated with Food Poisoning Outbreaks in British Columbia, Canada. Appl. Environ. Microbiol. 74: 7451-7453 [Abstract] [Full Text]  
  • Swiecicka, I., Bideshi, D. K., Federici, B. A. (2008). Novel Isolate of Bacillus thuringiensis subsp. thuringiensis That Produces a Quasicuboidal Crystal of Cry1Ab21 Toxic to Larvae of Trichoplusia ni. Appl. Environ. Microbiol. 74: 923-930 [Abstract] [Full Text]  
  • de Been, M., Francke, C., Moezelaar, R., Abee, T., Siezen, R. J. (2006). Comparative analysis of two-component signal transduction systems of Bacillus cereus, Bacillus thuringiensis and Bacillus anthracis.. Microbiology 152: 3035-3048 [Abstract] [Full Text]  
  • Daffonchio, D., Raddadi, N., Merabishvili, M., Cherif, A., Carmagnola, L., Brusetti, L., Rizzi, A., Chanishvili, N., Visca, P., Sharp, R., Borin, S. (2006). Strategy for Identification of Bacillus cereus and Bacillus thuringiensis Strains Closely Related to Bacillus anthracis. Appl. Environ. Microbiol. 72: 1295-1301 [Abstract] [Full Text]  
  • Zahner, V., Cabral, D. A., Regua-Mangia, A. H., Rabinovitch, L., Moreau, G., McIntosh, D. (2005). Distribution of Genes Encoding Putative Virulence Factors and Fragment Length Polymorphisms in the vrrA Gene among Brazilian Isolates of Bacillus cereus and Bacillus thuringiensis. Appl. Environ. Microbiol. 71: 8107-8114 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»