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Journal of Clinical Microbiology, August 2004, p. 3766-3774, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3766-3774.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Use of DNA Microarrays for Rapid Genotyping of TEM Beta-Lactamases That Confer Resistance

Verena Grimm,1 Satoshi Ezaki,1 Milorad Susa,2 Cornelius Knabbe,2 Rolf. D. Schmid,1 and Till T. Bachmann1*

Institute of Technical Biochemistry, University of Stuttgart,1 Department of Clinical Chemistry and Laboratory Medicine, Robert Bosch Hospital, Stuttgart, Germany2

Received 14 January 2004/ Returned for modification 15 March 2004/ Accepted 5 May 2004

Standard clinical procedures for pathogen resistance identification are laborious and usually require 2 days of cultivation before the resistance can be determined unequivocally. In contrast, clinicians and patients face increasing threats from antibiotic-resistant pathogenic bacteria in terms of their frequencies and levels of resistance. A major class of microbial resistance stems from the occurrence of beta-lactamases, which, if mutated, can cause the severe extended-spectrum beta-lactamase (ESBL) or inhibitor-resistant TEM (IRT) phenotype, which cause resistance to extended-spectrum cephalosporins, monobactams, and beta-lactamase inhibitors. We describe an oligonucleotide microarray for identification of the single nucleotide polymorphisms (SNPs) of 96% of the TEM beta-lactamase variants described to date which are related to the ESBL and/or IRT phenotype. The target DNA, originating from Escherichia coli, Enterobacter cloacae, and Klebsiella pneumoniae cells isolated from clinical samples, was amplified and fluorescently labeled by PCR with consensus primers in the presence of cyanine 5-labeled nucleotides. The total assay, including PCR, hybridization, and image analysis, could be performed in 3.5 h. The microarray results were validated by standard clinical procedures. The microarray outperformed the standard procedures in terms of assay time and the depth of information provided. In conclusion, this array offers an attractive option for the identification and epidemiologic monitoring of TEM beta-lactamases in the routine clinical diagnostic laboratory.

* Corresponding author. Mailing address: Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, Stuttgart, Germany. Phone: 49 (0) 711 685 3197. Fax: 49 (0) 711 685 3196. E-mail: itbtba{at}po.uni-stuttgart.de.

Journal of Clinical Microbiology, August 2004, p. 3766-3774, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3766-3774.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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