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Journal of Clinical Microbiology, August 2004, p. 3795-3798, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3795-3798.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Assessment of a New Selective Chromogenic Bacillus cereus Group Plating Medium and Use of Enterobacterial Autoinducer of Growth for Cultural Identification of Bacillus Species

R. Reissbrodt,1 A. Raßbach,2 B. Burghardt,1 I. Rienäcker,1 H. Mietke,3 J. Schleif,3 H. Tschäpe,1 M. Lyte,4 and P. H. Williams5*

Robert Koch-Institut, Wernigerode,1 Bundesforschungsanstalt für Viruskrankheiten der Tiere, Jena,2 Sächsische Landesanstalt für Lanwirtschaft, Leipzig, Germany,3 Department of Surgery, Minneapolis Medical Research Foundation/Hennepin County Medical Center, Minneapolis, and Department of Biological Sciences, Minnesota State University at Mankato, Mankato, Minnesota,4 Department of Microbiology & Immunology, University of Leicester, Leicester, United Kingdom5

Received 8 December 2003/ Returned for modification 5 April 2004/ Accepted 20 April 2004

A new chromogenic Bacillus cereus group plating medium permits differentiation of pathogenic Bacillus species by colony morphology and color. Probiotic B. cereus mutants were distinguished from wild-type strains by their susceptibilities to penicillin G or cefazolin. The enterobacterial autoinducer increased the sensitivity and the speed of enrichment of B. cereus and B. anthracis spores in serum-supplemented minimal salts medium (based on the standard American Petroleum Institute medium) and buffered peptone water.

* Corresponding author. Present address: Department of Genetics, University of Leicester, University Rd., Leicester LE1 7RH, United Kingdom. Phone: 44 116 252 3436. Fax: 44 116 252 3378. E-mail: phw2{at}le.ac.uk.

Journal of Clinical Microbiology, August 2004, p. 3795-3798, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3795-3798.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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