JCM Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wightman, F.
Right arrow Articles by Lewin, S. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wightman, F.
Right arrow Articles by Lewin, S. R.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 2004, p. 3809-3812, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3809-3812.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Comparison of Sequence Analysis and a Novel Discriminatory Real-Time PCR Assay for Detection and Quantification of Lamivudine-Resistant Hepatitis B Virus Strains

Fiona Wightman,1 Tomos Walters,2 Anna Ayres,2 Scott Bowden,2 Angeline Bartholomeusz,2 Daryl Lau,3 Stephen Locarnini,2 and Sharon R. Lewin1,4*

Department of Medicine, Monash University,1 Infectious Diseases Unit, The Alfred Hospital, Melbourne,4 Victorian Infectious Diseases Reference Laboratory, North Melbourne, Victoria, Australia,2 Department of Medicine, The University of Texas Medical Branch at Galveston, Galveston, Texas3

Received 10 November 2003/ Returned for modification 15 December 2003/ Accepted 26 January 2004

We report a rapid and accurate real-time PCR-based method to quantify wild-type and lamivudine-resistant hepatitis B virus by using a common forward primer paired with different reverse primers. Excellent concordance was demonstrated between sequencing and the discriminatory real-time assay; however, a mixture of quasispecies was more frequently detected by discriminatory real-time PCR.

* Corresponding author. Mailing address: Infectious Diseases Unit, Alfred Hospital, P.O. Box 315, Prahran, Victoria 3181, Australia. Phone: 613 9276 3009. Fax: 613 9276 2431. E-mail: s.lewin{at}alfred.org.au.

Journal of Clinical Microbiology, August 2004, p. 3809-3812, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3809-3812.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2004 by the American Society for Microbiology. All rights reserved.