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Journal of Clinical Microbiology, August 2004, p. 3813-3815, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3813-3815.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Detection of Haemophilus influenzae Type b by Real-Time PCR

Adrian Marty,1 Oliver Greiner,1 Philip J. R. Day,1,2 Sibylle Gunziger,1 Kathrin Mühlemann,3 and David Nadal1*

Division of Infectious Diseases,1 Division of Oncology, University Children's Hospital of Zurich, CH-8032 Zurich,2 Institute of Medical Microbiology, University of Berne, CH-3010 Berne, Switzerland3

Received 22 January 2004/ Returned for modification 24 February 2004/ Accepted 30 April 2004

A real-time PCR assay targeting the capsulation locus of Haemophilus influenzae type b (Hib) was developed. The linear detection range was from 1 to 106 microorganisms per reaction mixture. No H. influenzae other than Hib or any other control bacteria typically found in the upper respiratory tract was detected.

* Corresponding author. Mailing address: Division of Infectious Diseases, University Children's Hospital of Zurich, Steinwiesstr. 75, CH-8032 Zurich, Switzerland. Phone: 41-1-266-7562. Fax: 41-1-266-7157. E-mail: david.nadal{at}kispi.unizh.ch.

Journal of Clinical Microbiology, August 2004, p. 3813-3815, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3813-3815.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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