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Journal of Clinical Microbiology, August 2004, p. 3819-3822, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3819-3822.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Differentiation of the Major Listeria monocytogenes Serovars by Multiplex PCR

Michel Doumith,1* Carmen Buchrieser,2 Philippe Glaser,2 Christine Jacquet,1 and Paul Martin1

Laboratoire des Listeria-Centre National de Référence des Listeria-World Health Organization Collaborating Center for Foodborne Listeriosis,1 Laboratoire de Génomique des Microorganismes Pathogènes, Institut Pasteur, 75724 Paris Cedex 15, France2

Received 31 October 2003/ Returned for modification 5 January 2004/ Accepted 20 April 2004

A new multiplex PCR assay was developed to separate the four major Listeria monocytogenes serovars isolated from food and patients (1/2a, 1/2b, 1/2c, and 4b) into distinct groups. The PCR test, which constitutes a rapid and practical alternative to laborious classical serotyping, was successfully evaluated with 222 Listeria strains.

* Corresponding author. Mailing address: Laboratoire des Listeria-Centre National de Référence des Listeria-World Health Organization Collaborating Center for Foodborne Listeriosis, Institut Pasteur, 25 Rue du Dr. Roux, 75724 Paris Cedex 15, France. Phone: (33-1)-40-61-31-12. Fax: (33-1)-40-61-35-67. E-mail: mdoumith{at}pasteur.fr.

Journal of Clinical Microbiology, August 2004, p. 3819-3822, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3819-3822.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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