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Journal of Clinical Microbiology, August 2004, p. 3873-3876, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3873-3876.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Distribution of Porphyromonas gingivalis Biotypes Defined by Alleles of the kgp (Lys-Gingipain) Gene

Mangala A. Nadkarni,1* Ky-Anh Nguyen,1 Cheryl C. Chapple,1 Arthur A. DeCarlo,2,3 Nicholas A. Jacques,1 and Neil Hunter1

Institute of Dental Research, Westmead Millennium Institute, and Westmead Centre for Oral Health,1 United Dental Hospital, Sydney, Australia,2 NSU Dental, Nova Southeastern University, Ft. Lauderdale, Florida3

Received 12 November 2003/ Returned for modification 3 February 2004/ Accepted 28 April 2004

Paired subgingival plaque samples representing the most-diseased and least-diseased sites were collected from 34 adult patients with diagnosed chronic periodontitis. The percentage of Porphyromonas gingivalis relative to the total anaerobic and gram-negative bacterial load at each site was determined by real-time PCR. Based on variations in the noncatalytic C terminus of the Lys-gingipain (Kgp), it was reasoned that DNA sequence variation in the 3'-coding region of the kgp gene might determine functional biotypes. Perusal of the available sequence information in GenBank indicated three such forms of the kgp gene corresponding to P. gingivalis strains HG66, 381, and W83. Analysis of patient samples revealed the presence of a fourth genotype (W83v) that showed duplication of a sequence recognized by the W83 reverse primer. The four biotypes, HG66, 381, W83, and W83v, were present in the study group in the ratio 8:11:6:5, respectively. Each subject was colonized by one predominant biotype, and only three patients were colonized by a trace amount of a second biotype.

* Corresponding author. Mailing address: Institute of Dental Research, Westmead Centre for Oral Health, P.O. Box 533, Wentworthville, NSW 2145, Australia. Phone: 61 2 9845 7826. Fax: 61 2 9845 7599. E-mail: mnadkarni{at}dental.wsahs.nsw.gov.au.

Journal of Clinical Microbiology, August 2004, p. 3873-3876, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3873-3876.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nadkarni, M. A., Chhour, K.-L., Browne, G., Jacques, N. A., Hunter, N. (2009). Lysine Gingipain (kgp) Biovars of Porphyromonas gingivalis Exhibit Differential Distribution on Oral Mucosal Sites. J. Clin. Microbiol. 47: 3350-3352 [Abstract] [Full Text]  


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