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Comprehensive Detection and Serotyping of Human Adenoviruses by PCR and Sequencing

Helen Sarantis,¹ Grant Johnson,¹ Martha Brown,² Martin Petric,^1,3, and Raymond Tellier^1,3*

Division of Microbiology, Hospital for Sick Children,¹ Department of Laboratory Medicine and Pathobiology,³ Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada²

Received 10 June 2003/ Returned for modification 3 August 2003/ Accepted 9 May 2004

Human adenoviruses are common pathogens associated with many diseases, including respiratory, gastrointestinal, and ocular infections. Because they are now being increasingly recognized as agents of life-threatening disseminated infection in immunocompromised patients, robust and sensitive laboratory detection methods are needed for their rapid diagnosis. We describe here a PCR assay using a single primer pair, targeting a region of the hexon gene containing hypervariable region 7, that can detect all known human adenovirus serotypes and allows for serotype determination through the analysis of the nucleotide sequence. This comprehensive assay has proven effective for diagnosing adenoviruses at the serotype level in a broad range of patient specimens, including conjunctival, nasopharyngeal, stool, blood, and urine specimens.

Present address: British Columbia Center for Disease Control, Vancouver, British Columbia, Canada.

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