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Journal of Clinical Microbiology, September 2004, p. 4025-4031, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4025-4031.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Microevolution of Candida albicans Strains during Catheter-Related Candidemia

Jong Hee Shin,^1* Mi-Ra Park,¹ Jeong Won Song,¹ Dong Hyeon Shin,² Sook-In Jung,² Duck Cho,¹ Seung Jung Kee,¹ Myung Geun Shin,¹ Soon Pal Suh,¹ and Dong Wook Ryang¹

Departments of Laboratory Medicine,¹ Internal Medicine, Chonnam National University Medical School, Gwangju, Korea²

Received 20 April 2004/ Returned for modification 4 May 2004/ Accepted 21 May 2004

We examined microevolution in a series of Candida albicans strains isolated from patients with catheter-related candidemia. Sixty-one isolates (29 from blood, 18 from catheters, 10 from urine, and 4 from other sites) were obtained from 15 patients who were admitted to the same hospital over a 3-year period. Isolates were analyzed by using Southern hybridization with the C1 fragment of Ca3 as a probe (C1 fingerprinting) and pulsed-field gel electrophoresis (PFGE). PFGE typing consisted of electrophoretic karyotyping (EK) and restriction endonuclease analysis of genomic DNA (REAG) by using SfiI (REAG-S) and BssHII (REAG-B). When catheter isolates were compared with blood isolates from the same patient, catheter isolates from 5 of 14 patients (36%) exhibited minor band differences (microevolution) relative to blood isolates in either C1 fingerprinting (n = 4), REAG-S (n = 3), or REAG-B (n = 5) profiles, although they had identical EK patterns. However, the other sequential isolates from each patient, which had identical EK patterns, showed the same REAG and C1 fingerprinting patterns. Both fingerprinting methods revealed that two distinct genotypes were shared by isolates from seven patients in a neonatal intensive care unit, suggesting two nosocomial clusters. Except for two catheter isolates from the index patients of each cluster, no consecutive isolates collected from each of the two clusters showed any microevolution during the 2- or 7-month cluster periods. The findings suggest that in catheter-related candidemia, some C. albicans strains undergo microevolution during catheter colonization.

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* Corresponding author. Mailing address: Department of Laboratory Medicine, Chonnam National University Medical School, 8 Hakdong Dongku, Gwangju 501-757, South Korea. Phone: 82 (62) 220-5342. Fax: 82 (62) 224-2518. E-mail: shinjh{at}chonnam.ac.kr.

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Journal of Clinical Microbiology, September 2004, p. 4025-4031, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4025-4031.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

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