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Journal of Clinical Microbiology, September 2004, p. 4067-4071, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4067-4071.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Evaluation of the New VITEK 2 Card for Identification of Clinically Relevant Gram-Negative Rods

Guido Funke* and Pascale Funke-Kissling

Department of Medical Microbiology and Hygiene, Gärtner & Colleagues Laboratories, Weingarten, Germany

Received 27 February 2004/ Returned for modification 13 May 2004/ Accepted 17 May 2004

The VITEK 2 card for gram-negative bacteria (bioMérieux,Marcy-l'Etoile, France) has been redesigned to improve the identification of fermenting and nonfermenting bacilli. Forty-seven biochemical tests, including 19 enzymatic tests, are present in the new card and interpreted in a kinetic mode. Final identification results are available within 10 h. The database allows the identification of 159 different taxa. Six hundred fifty-five gram-negative rods (GNR; 511 fermenters and 144 nonfermenters), representing 54 taxa, were tested. Strains were taken from fresh routine primary isolation plates (n = 157), from stored routine plates (n = 301), and from stock cultures (n = 197). Six hundred thirty-seven strains (97.3%) were correctly identified to the species level, 14 strains (2.1%) gave low discrimination results requiring additional tests, and 4 strains (0.6%) gave discordant results; not a single strain remained unidentified. Nearly 92% of all isolates were correctly identified within 7 h of incubation. The robustness of the system was demonstrated by the fact that strains were grown on four different agar media before testing. The system may also have the potential to be applied directly to primary isolation plates, since in this instance 96.2% of 157 GNR were correctly identified and 3.8% gave low discrimination results. The new VITEK 2 card for gram-negative bacteria seems to be a promising new tool for routine, rapid identification of GNR.

* Corresponding author. Mailing address: Department of Medical Microbiology and Hygiene, Gärtner & Colleagues Laboratories, Hoyerstrasse 51, D-88250 Weingarten, Germany. Phone: 49-751-502-630. Fax: 49-751-502-385. E-mail: ldg.funke{at}t-online.de.

Journal of Clinical Microbiology, September 2004, p. 4067-4071, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4067-4071.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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