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Journal of Clinical Microbiology, September 2004, p. 4164-4168, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4164-4168.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

A Predominant and Virulent Legionella pneumophila Serogroup 1 Strain Detected in Isolates from Patients and Water in Queensland, Australia, by an Amplified Fragment Length Polymorphism Protocol and Virulence Gene-Based PCR Assays

Bixing Huang,1* Brett A. Heron,1 Bruce R. Gray,1 Sofroni Eglezos,2 John R. Bates,1 and John Savill1

Public Health Microbiology Laboratory, Queensland Health Scientific Services, Coopers Plains,1 EML Consulting Services Queensland Pty. Ltd., Tennyson, Queensland, Australia.2

Received 26 March 2004/ Returned for modification 9 May 2004/ Accepted 6 June 2004

In epidemiological investigations of community legionellosis outbreaks, knowledge of the prevalence, distribution, and clinical significance (virulence) of environmental Legionella isolates is crucial for interpretation of the molecular subtyping results. To obtain such information for Legionella pneumophila serogroup 1 isolates, we used the standardized amplified fragment length polymorphism (AFLP) protocol of the European Working Group on Legionella Infection to subtype L. pneumophila SG1 isolates obtained from patients and water sources in Queensland, Australia. An AFLP genotype, termed AF1, was predominant in isolates from both patients (40.5%) and water (49.0%). The second most common AFLP genotype found in water isolates was AF16 (36.5%), but this genotype was not identified in the patient isolates. When virulence gene-based PCR assays for lvh and rtxA genes were applied to the isolates from patients and water, nearly all (65 of 66) AF1 strains had both virulence genes, lvh and rtxA. In contrast, neither the lvh nor the rtxA gene was found in the AF16 strains, except for one isolate with the rtxA gene. It appears that this may explain the failure to find this genotype in the isolates from patients even though it may be common in the environment. In view of the evidence that the AF1 genotype is the most common genotype among strains found in patients and water sources in this region, any suggested epidemiological link derived from comparing the AF1 genotype from patient isolates with the AF1 genotype from environmental isolates must be interpreted and acted on with caution. The use of virulence gene-based PCR assays applied to environmental samples may be helpful in determining the infection potential of the isolates involved.

* Corresponding author. Mailing address: Molecular Microbiology R&D Unit, Public Health Microbiology Laboratory, Queensland Health Scientific Services, 39 Kessels Rd., Coopers Plains, QLD 4108, Australia. Phone: 64 7 32749069. Fax: 64 7 32749175. E-mail: Ben_Huang{at}health.qld.gov.au.

Journal of Clinical Microbiology, September 2004, p. 4164-4168, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4164-4168.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Huang, B., Yuan, Z., Heron, B. A., Gray, B. R., Eglezos, S., Bates, J. R., Savill, J. (2006). Distribution of 19 major virulence genes in Legionella pneumophila serogroup 1 isolates from patients and water in Queensland, Australia.. J Med Microbiol 55: 993-997 [Abstract] [Full Text]  
  • van der Mee-Marquet, N., Domelier, A.-S., Arnault, L., Bloc, D., Laudat, P., Hartemann, P., Quentin, R. (2006). Legionella anisa, a Possible Indicator of Water Contamination by Legionella pneumophila. J. Clin. Microbiol. 44: 56-59 [Abstract] [Full Text]  
  • Wullings, B. A., van der Kooij, D. (2006). Occurrence and Genetic Diversity of Uncultured Legionella spp. in Drinking Water Treated at Temperatures below 15{degrees}C. Appl. Environ. Microbiol. 72: 157-166 [Abstract] [Full Text]  


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