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Journal of Clinical Microbiology, September 2004, p. 4250-4252, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4250-4252.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Whole-Blood Counting Immunoassay as a Short-Turnaround Test for Detection of Hepatitis B Surface Antigen, Anti-Hepatitis C Virus Antibodies, and Anti-Treponema pallidum Antibodies

Toyoichiro Kudo,1* Aiko Kido,1 Yukiko Nishiyama,1 Hiroshi Koganeya,1 Takako Okuda,1 Motoshige Nabeshima,2 Yoshitsugu Iinuma,1 and Satoshi Ichiyama1

Department of Laboratory Medicine,1 Department of Gastroenterology and Hepatology, Graduate School of Medicine, Kyoto University, Kyoto, Japan2

Received 21 October 2003/ Returned for modification 7 January 2004/ Accepted 6 May 2004

Whole-blood samples were used for a counting immunoassay (CIA) with the aim of developing a short- turnaround test. After optimization of the CIA, hepatitis B surface antigen (HBsAg), anti-hepatitis C virus antibodies (anti-HCV), and anti-Treponema pallidum antibodies (anti-TP) were detected as efficiently as by an enzyme immunoassay (EIA) with serum samples. The correlations between whole-blood CIA and serum EIA were 99.8, 97.1, and 99.4% for HBsAg, anti-HCV, and anti-TP, respectively. Whole-blood CIA may be of value when rapid screening of many samples is required.

* Corresponding author. Mailing address: Clinical Laboratory, Division of Infectious Diseases, Kyoto University Hospital, 54 Kawahara Shogoin, Sakyoku, Kyoto 606-8507, Japan. Phone: 81-75-751-4914. Fax: 81-75-751-3233. E-mail: tkudo{at}kuhp.kyoto-u.ac.jp.

Journal of Clinical Microbiology, September 2004, p. 4250-4252, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4250-4252.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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