This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gupta, A. K. Articles by Batra, R. Search for Related Content PubMed PubMed Citation Articles by Gupta, A. K. Articles by Batra, R.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, September 2004, p. 4253-4260, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4253-4260.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Identification and Typing of Malassezia Species by Amplified Fragment Length Polymorphism and Sequence Analyses of the Internal Transcribed Spacer and Large-Subunit Regions of Ribosomal DNA

Division of Dermatology, Department of Medicine, Sunnybrook and Women's College Health Science Center, the University of Toronto, Toronto,¹ Mediprobe Laboratories, London, Ontario, Canada,² Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands³

Received 8 January 2004/ Returned for modification 3 March 2004/ Accepted 28 March 2004

Malassezia yeasts are associated with several dermatological disorders. The conventional identification of Malassezia species by phenotypic methods is complicated and time-consuming, and the results based on culture methods are difficult to interpret. A comparative molecular approach based on the use of three molecular techniques, namely, amplified fragment length polymorphism (AFLP) analysis, sequencing of the internal transcribed spacer, and sequencing of the D1 and D2 domains of the large-subunit ribosomal DNA region, was applied for the identification of Malassezia species. All species could be correctly identified by means of these methods. The results of AFLP analysis and sequencing were in complete agreement with each other. However, some discrepancies were noted when the molecular methods were compared with the phenotypic method of identification. Specific genotypes were distinguished within a collection of Malassezia furfur isolates from Canadian sources. AFLP analysis revealed significant geographical differences between the North American and European M. furfur strains.

This article has been cited by other articles:

• Wang, Q.-M., Li, J., Wang, S.-A., Bai, F.-Y. (2008). Rapid Differentiation of Phenotypically Similar Yeast Species by Single-Strand Conformation Polymorphism Analysis of Ribosomal DNA. Appl. Environ. Microbiol. 74: 2604-2611 [Abstract] [Full Text]  
• Diaz, M. R., Boekhout, T., Theelen, B., Bovers, M., Cabanes, F. J., Fell, J. W. (2006). Microcoding and flow cytometry as a high-throughput fungal identification system for Malassezia species.. J Med Microbiol 55: 1197-1209 [Abstract] [Full Text]  
• Paulino, L. C., Tseng, C.-H., Strober, B. E., Blaser, M. J. (2006). Molecular analysis of fungal microbiota in samples from healthy human skin and psoriatic lesions.. J. Clin. Microbiol. 44: 2933-2941 [Abstract] [Full Text]  
• Playford, E. G., Kong, F., Sun, Y., Wang, H., Halliday, C., Sorrell, T. C. (2006). Simultaneous detection and identification of Candida, Aspergillus, and cryptococcus species by reverse line blot hybridization.. J. Clin. Microbiol. 44: 876-880 [Abstract] [Full Text]