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Journal of Clinical Microbiology, September 2004, p. 4293-4296, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4293-4296.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Simple Chemical Extraction Method for DNA Isolation from Aspergillus fumigatus and Other Aspergillus Species

J. Jin, Y.-K. Lee, and B. L. Wickes*

Department of Microbiology and Immunology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas

Received 17 October 2003/ Returned for modification 20 January 2004/ Accepted 13 May 2004

DNA was successfully isolated from numerous Aspergillus spp. by use of a commercial kit. DNA that was easily digested and yielded PCR products up to 8.5 kb in size was recovered from broth or agar cultures. The ease and speed of this protocol provide an alternative to physical methods of DNA isolation.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229. Phone: (210) 567-3938. Fax: (210) 567-6612. E-mail: wickes{at}uthscsa.edu.

Journal of Clinical Microbiology, September 2004, p. 4293-4296, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4293-4296.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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