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Journal of Clinical Microbiology, September 2004, p. 4307-4309, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4307-4309.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Comparison of the Real-Time PCR Method and the Gen-Probe Amplified Mycobacterium tuberculosis Direct Test for Detection of Mycobacterium tuberculosis in Pulmonary and Nonpulmonary Specimens

Nadine Lemaître,1* Sylvie Armand,1 Anne Vachée,2 Odile Capilliez,1 Christine Dumoulin,1 and René J. Courcol1

Laboratoire de Bactériologie-Hygiène, Centre Hospitalier Régional Universitaire de Lille, Lille,1 Laboratoire de Biologie Polyvalente, Hôpital de Roubaix, Roubaix, France2

Received 24 February 2004/ Returned for modification 18 March 2004/ Accepted 4 May 2004

Real-time PCR was compared to Amplified Mycobacterium tuberculosis Direct Test (AMTDII) for 100 clinical specimens. The overall sensitivities of the real-time PCR method and AMTDII were similar for respiratory and nonrespiratory specimens. However, real-time PCR seemed to be less susceptible to amplification inhibitors than AMTDII.

* Corresponding author. Mailing address: Laboratoire de Bactériologie-Hygiène, Hôpital A. Calmette, CHRU de Lille, Bvd. du Professeur Jules Leclercq, 59037 Lille Cedex, France. Phone: (33) 3 20 44 45 92. Fax: (33) 3 20 44 48 95. E-mail: n-lemaitre{at}chru-lille.fr.

Journal of Clinical Microbiology, September 2004, p. 4307-4309, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4307-4309.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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