This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kilpatrick, D. R.
Right arrow Articles by Kew, O. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kilpatrick, D. R.
Right arrow Articles by Kew, O. M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, September 2004, p. 4313-4315, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4313-4315.2004

Multiplex PCR Method for Identifying Recombinant Vaccine-Related Polioviruses

David R. Kilpatrick,* Karen Ching, Jane Iber, Ray Campagnoli, Christopher J. Freeman, Nada Mishrik, Hong-Mei Liu, Mark A. Pallansch, and Olen M. Kew

Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

Received 9 March 2004/ Returned for modification 21 April 2004/ Accepted 13 May 2004

The recent discovery of recombinant circulating vaccine-derived poliovirus (recombinant cVDPV) has highlighted the need for enhanced global poliovirus surveillance to assure timely detection of any future cVDPV outbreaks. Six pairs of Sabin strain-specific recombinant primers were designed to permit rapid screening for VDPV recombinants by PCR.

* Corresponding author. Mailing address: Respiratory and Enteric Virus Branch, G-10, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333. Phone: (404) 639-1345. Fax: (404) 639-4011. E-mail: DKilpatrick{at}cdc.gov.

Journal of Clinical Microbiology, September 2004, p. 4313-4315, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4313-4315.2004




This article has been cited by other articles:

  • Kilpatrick, D. R., Yang, C.-F., Ching, K., Vincent, A., Iber, J., Campagnoli, R., Mandelbaum, M., De, L., Yang, S.-J., Nix, A., Kew, O. M. (2009). Rapid Group-, Serotype-, and Vaccine Strain-Specific Identification of Poliovirus Isolates by Real-Time Reverse Transcription-PCR Using Degenerate Primers and Probes Containing Deoxyinosine Residues. J. Clin. Microbiol. 47: 1939-1941 [Abstract] [Full Text]  
  • Yang, C.-F., Chen, H.-Y., Jorba, J., Sun, H.-C., Yang, S.-J., Lee, H.-C., Huang, Y.-C., Lin, T.-Y., Chen, P.-J., Shimizu, H., Nishimura, Y., Utama, A., Pallansch, M., Miyamura, T., Kew, O., Yang, J.-Y. (2005). Intratypic Recombination among Lineages of Type 1 Vaccine-Derived Poliovirus Emerging during Chronic Infection of an Immunodeficient Patient. J. Virol. 79: 12623-12634 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»