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Journal of Clinical Microbiology, September 2004, p. 4344-4348, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4344-4348.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Detection and Identification of Actinobacillus pleuropneumoniae Serotypes 1, 2, and 8 by Multiplex PCR

Jennifer A. Schuchert,1 T. J. Inzana,1* Ø. Angen,2 and S. Jessing2

Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia,1 Danish Veterinary Institute, Copenhagen, Denmark2

Received 8 October 2003/ Returned for modification 16 February 2004/ Accepted 20 May 2004

Multiplex PCR assays were developed to identify Actinobacillus pleuropneumoniae serotypes 1, 2, and 8. Primers designed for the conserved capsular polysaccharide (CP) export region amplified a 489-bp DNA fragment from all serotypes. Primers specific to the CP biosynthesis regions of serotypes 1, 2, and 8 amplified fragments of 1.6 kb, 1.7 kb, and 970 bp from only their respective serotypes.

* Corresponding author. Mailing address: Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, 1410 Prices Fork Rd., Virginia Polytechnic Institute and State University, Blacksburg, VA 24061-0342. Phone: (540) 231-4692. Fax: (540) 231-3426. E-mail: tinzana{at}vt.edu.

Journal of Clinical Microbiology, September 2004, p. 4344-4348, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4344-4348.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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