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Journal of Clinical Microbiology, September 2004, p. 4361-4364, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4361-4364.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Development of a Real-Time PCR Assay Using SYBR Green I for Provirus Load Quantification in a Murine Model of AIDS

Anna Casabianca, Chiara Orlandi, Alessandra Fraternale, and Mauro Magnani*

Institute of Biological Chemistry "Giorgio Fornaini," University of Urbino, Urbino, Italy

Received 5 December 2003/ Returned for modification 4 May 2004/ Accepted 23 May 2004

A real-time PCR assay using SYBR Green I for quantification of provirus load in a murine model of AIDS (i.e., LP-BM5 infection) was developed and validated. In this method, data are normalized against the 18S rRNA gene. The method has a dynamic range of 8 logs and a sensitivity of one copy.

* Corresponding author. Mailing address: Istituto di Chimica Biologica "G. Fornaini," Università degli Studi di Urbino, Via Saffi, 2 61029 Urbino (PU), Italy. Phone: 39-0722-305211. Fax: 39-0722-320188. E-mail: magnani{at}uniurb.it.

Journal of Clinical Microbiology, September 2004, p. 4361-4364, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4361-4364.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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