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Journal of Clinical Microbiology, January 2005, p. 112-119, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.112-119.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Preclinical Diagnosis of African Swine Fever in Contact-Exposed Swine by a Real-Time PCR Assay

L. Zsak,1* M. V. Borca,1 G. R. Risatti,1 A. Zsak,1 R. A. French,2 Z. Lu,1 G. F. Kutish,1 J. G. Neilan,1 J. D. Callahan,3 W. M. Nelson,3 and D. L. Rock1

Plum Island Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Greenport, New York,1 Department of Pathobiology, University of Connecticut, Storrs, Connecticut,2 Tetracore Inc., Gaithersburg, Maryland3

Received 2 March 2004/ Returned for modification 26 July 2004/ Accepted 6 September 2004

A fluorogenic probe hydrolysis (TaqMan) PCR assay for African swine fever virus (ASFV) was developed and evaluated in experimentally infected swine. This sensitive and specific one-step single-tube assay, which can be performed in 2 h or less, detected viral DNA in tonsil scraping samples 2 to 4 days prior to onset of clinical disease. Thus, the assay would have application for preclinical diagnosis of African swine fever and surveillance and/or emergency management of a disease outbreak.

* Corresponding author. Mailing address: Plum Island Animal Disease Center, P.O. Box 848, Greenport, NY 11944-0848. Phone: (631) 323-3023. Fax: (631) 323-2507. E-mail: lzsak{at}piadc.ars.usda.gov.

Journal of Clinical Microbiology, January 2005, p. 112-119, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.112-119.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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