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Journal of Clinical Microbiology, January 2005, p. 150-155, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.150-155.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Comparison of emm Typing and Ribotyping with Three Restriction Enzymes To Characterize Clinical Isolates of Streptococcus pyogenes

Stella Z. Doktor,* Jill M. Beyer, Robert K. Flamm,{dagger} and Virginia D. Shortridge

Infectious Diseases Research, Abbott Laboratories, Abbott Park, Illinois

Received 25 June 2004/ Returned for modification 17 August 2004/ Accepted 15 September 2004

A total of 336 Streptococcus pyogenes isolates recently recovered from patients with pharyngitis from 13 countries were characterized by emm typing and riboprinting using an automated Riboprinter (Dupont/Qualicon) based on the patterns produced by three restriction enzymes, EcoRI, PstI, and HindIII. Three enzymes were necessary to increase the discrimination of ribogroups formed by each enzyme. A total of 40 ribogroups and 38 emm sequences (not counting allelic variations) were identified. Multilocus sequence typing was performed on a sampling of the isolates, and those results were consistent with those of both emm typing and ribotyping. Correlations were observed among all three methods.

* Corresponding author. Mailing address: AP52N, Dept. R47T, 200 Abbott Park Rd., Abbott Park, IL 60064. Phone: (847) 935-8044. Fax: (847) 935-0400. E-mail: stella.z.doktor{at}abbott.com.

{dagger} Present address: Focus Technologies, Herndon, VA 20171.

Journal of Clinical Microbiology, January 2005, p. 150-155, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.150-155.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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