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Journal of Clinical Microbiology, January 2005, p. 214-222, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.214-222.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Rapid, High-Throughput, Multiplex, Real-Time PCR for Identification of Mutations in the cyp51A Gene of Aspergillus fumigatus That Confer Resistance to Itraconazole

Public Health Research Institute, International Center for Public Health,¹ Department of Microbiology and Molecular Genetics, New Jersey Medical School-University of Medicine and Dentistry of New Jersey, Newark, New Jersey²

Received 29 July 2004/ Returned for modification 2 September 2004/ Accepted 24 September 2004

Aspergillus fumigatus is an important cause of life-threatening invasive fungal disease in patients with compromised immune systems. Resistance to itraconazole in A. fumigatus is closely linked to amino acid substitutions in Cyp51A that replace Gly54. In an effort to develop a new class of molecular diagnostic assay that can rapidly assess drug resistance, a multiplexed assay was established. This assay uses molecular beacons corresponding to the wild-type cyp51A gene and seven mutant alleles encoding either Arg54, Lys54, Val54, Trp54, or Glu54. Molecular beacon structure design and real-time PCR conditions were optimized to increase the assay specificity. The multiplex assay was applied to the analysis of chromosomal DNA samples from a collection of 48 A. fumigatus clinical and laboratory-derived isolates, most with reduced susceptibility to itraconazole. The cyp51A allelic identities for codon 54 were established for all of the strains tested, and mutations altering Gly54 in 23 strains were revealed. These mutations included G₅₄W (n = 1), G₅₄E (n = 12), G₅₄K (n = 3), G₅₄R (n = 3), and G₅₄V (n = 4). Molecular beacon assay results were confirmed by DNA sequencing. Multiplex real-time PCR with molecular beacons is a powerful technique for allele differentiation and analysis of resistance mutations that is dynamic and suitable for rapid high-throughput assessment of drug resistance.

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