This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Christensen, H.
Right arrow Articles by Olsen, J. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Christensen, H.
Right arrow Articles by Olsen, J. E.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, January 2005, p. 259-270, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.259-270.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Characterization of Sucrose-Negative Pasteurella multocida Variants, Including Isolates from Large-Cat Bite Wounds

Henrik Christensen,1* Magne Bisgaard,1 Øystein Angen,2 Wilhelm Frederiksen,3 and John Elmerdahl Olsen1

Department of Veterinary Pathobiology, The Royal Danish Veterinary and Agricultural University, Frederiksberg,1 Danish Food and Veterinary Research, Copenhagen,2 Rødding Tværvej 3, Rødding, Denmark3

Received 16 July 2004/ Returned for modification 29 August 2004/ Accepted 12 September 2004

To validate the identification of Pasteurella multocida-like bacteria negative for acid formation from sucrose, including isolates from bite wounds caused by large cats, 17 strains were phenotypically and genotypically characterized. Phylogenetic analysis of partially sequenced rpoB and infB genes showed the monophyly of the strains characterized and the reference strains of P. multocida. The sucrose-negative strains formed two groups, one related to reference strains of P. multocida and the other related to a separate species-like group (taxon 45 of Bisgaard). DNA-DNA hybridization further documented the species-like nature of this group. Ribotyping showed the heterogeneity of all strains except four strains that shared the same ribotype and that were isolated from bovine lungs. Phylogenetic analysis by 16S rRNA sequence comparison showed the monophyly of the strains characterized and the reference strains of P. multocida. Two strains isolated from leopard bite wounds were related to the type strain of P. dagmatis; however, they represented a new taxon (taxon 46 of Bisgaard), in accordance with their distinct phenotypic and genotypic identifications. The present study documents that sucrose-negative strains of P. multocida-like bacteria belong to two genotypically distinct groups. The study further confirms the phenotypic heterogeneity of P. multocida strains and documents two new species-like taxa of Pasteurella related to P. multocida. Until diagnostic tools have been further elaborated, special care should be taken in the identification of Pasteurella-like bacteria isolated from bite wounds caused by large cats. The evidence of phenotypic and genotypic divergence calls for the further development of PCR tests and DNA sequencing to document doubtful isolates.

* Corresponding author. Mailing address: Department of Veterinary Pathobiology, The Royal Danish Veterinary and Agricultural University, Stigbøjlen 4, 1870 Frederiksberg C, Denmark. Phone: 45 35282783. Fax: 45 35282757. E-mail: hech{at}kvl.dk.

Journal of Clinical Microbiology, January 2005, p. 259-270, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.259-270.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»