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Journal of Clinical Microbiology, January 2005, p. 284-292, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.284-292.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Candida orthopsilosis and Candida metapsilosis spp. nov. To Replace Candida parapsilosis Groups II and III

Arianna Tavanti,1 Amanda D. Davidson,1 Neil A. R. Gow,1 Martin C. J. Maiden,2 and Frank C. Odds1*

Aberdeen Fungal Group, School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen,1 The Peter Medawar Building for Pathogen Research and Department of Zoology, University of Oxford, Oxford, United Kingdom2

Received 26 May 2004/ Returned for modification 29 June 2004/ Accepted 7 July 2004

Two new species, Candida orthopsilosis and C. metapsilosis, are proposed to replace the existing designations of C. parapsilosis groups II and III, respectively. The species C. parapsilosis is retained for group I isolates. Attempts to construct a multilocus sequence typing scheme to differentiate individual strains of C. parapsilosis instead revealed fixed DNA sequence differences between pairs of subgroups in four genes: COX3, L1A1, SADH, and SYA1. PCR amplicons for sequencing were obtained for these four plus a further seven genes from 21 group I isolates. For nine group II isolates, PCR products were obtained from only 5 of the 11 genes, and for two group III isolates PCR products were obtained from a different set of 5 genes. Three of the PCR products from group II and III isolates differed in size from the group I products. Cluster analysis of sequence polymorphisms from COX3, SADH, and SYA1, which were common to the three groups, consistently separated the isolates into three distinct sets. All of these differences, together with DNA sequence similarities <90% in the ITS1 sequence, suggest the subgroups should be afforded species status. The near absence of DNA sequence variability among isolates of C. parapsilosis and relatively high levels of sequence variability among isolates of C. orthopsilosis suggest that the former species may have evolved very recently from the latter.

* Corresponding author. Mailing address: Aberdeen Fungal Group, School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, United Kingdom. Phone: 44-1224-555828. Fax: 44-1224-555828. E-mail: f.odds{at}abdn.ac.uk.

Journal of Clinical Microbiology, January 2005, p. 284-292, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.284-292.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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