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Journal of Clinical Microbiology, January 2005, p. 340-347, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.340-347.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Sequence Typing and Comparison of Population Biology of Campylobacter coli and Campylobacter jejuni

Kate E. Dingle,1,2* Frances M. Colles,3 Daniel Falush,3 and Martin C. J. Maiden3

Nuffield Department of Clinical Sciences, Oxford University,1 Department of Microbiology, Oxford Radcliffe NHS Trust, Level 6 Microbiology, John Radcliffe Hospital,2 The Peter Medawar Building for Pathogen Research and Department of Zoology, University of Oxford, Oxford, United Kingdom3

Received 23 June 2004/ Returned for modification 17 August 2004/ Accepted 3 September 2004

A multilocus sequence typing (MLST) scheme that uses the same loci as a previously described system for Campylobacter jejuni was developed for Campylobacter coli. The C. coli-specific primers were validated with 53 isolates from humans, chickens, and pigs, together with 15 Penner serotype reference isolates. The nucleotide sequence of the flaA short variable region (SVR) was determined for each isolate. These sequence data were compared to equivalent information for 17 C. jejuni isolates representing the known genetic diversity of this species. C. coli and C. jejuni share approximately 86.5% identity at the nucleotide sequence level within the MLST loci. There is evidence of genetic exchange of the housekeeping genes between the two species, but at a very low rate; only one sequence type from each species showed evidence of imported DNA. The flaA gene was more variable and has been exchanged many times between the two species, making it an unreliable marker for species identification but useful for distinguishing closely related strains. All but 3 of 21 human C. coli clinical isolates were distinct, according to the combined MLST and SVR sequences. The use of a common MLST scheme allows direct comparisons of the population biology and molecular epidemiology of these two closely related human pathogens.

* Corresponding author. Mailing address: Nuffield Department of Clinical Sciences, Oxford University, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom. Phone: 44 1865 220870. Fax: 44 1865 764192. E-mail: kate.dingle{at}ndcls.ox.ac.uk.

Journal of Clinical Microbiology, January 2005, p. 340-347, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.340-347.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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