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Detection of Human Influenza A Viruses by Loop-Mediated Isothermal Amplification

Department of Microbiology, University of Hong Kong,¹ Department of Microbiology, Queen Mary Hospital, Hong Kong²

Received 6 June 2004/ Returned for modification 4 August 2004/ Accepted 28 August 2004

Here we describe the use of the loop-mediated isothermal amplification (LAMP) method to detect human influenza viruses (H1 to H3). Our results were correlated 100% with results deduced from routine clinical diagnostic tests. In addition, we also developed a LAMP assay specific for human ß-actin cDNA as a quality control test.

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