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Journal of Clinical Microbiology, January 2005, p. 427-430, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.427-430.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Detection of Human Influenza A Viruses by Loop-Mediated Isothermal Amplification

Leo L. M. Poon,1* Cynthia S. W. Leung,1 Kwok H. Chan,2 Jack H. C. Lee,1 Kwok Y. Yuen,1 Yi Guan,1 and Joseph S. M. Peiris1

Department of Microbiology, University of Hong Kong,1 Department of Microbiology, Queen Mary Hospital, Hong Kong2

Received 6 June 2004/ Returned for modification 4 August 2004/ Accepted 28 August 2004

Here we describe the use of the loop-mediated isothermal amplification (LAMP) method to detect human influenza viruses (H1 to H3). Our results were correlated 100% with results deduced from routine clinical diagnostic tests. In addition, we also developed a LAMP assay specific for human ß-actin cDNA as a quality control test.

* Corresponding author. Mailing address: Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR. Phone: (852) 2855 4384. Fax: (852) 2855 1241. E-mail: llmpoon{at}hkucc.hku.hk.

Journal of Clinical Microbiology, January 2005, p. 427-430, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.427-430.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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