This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Prakash, P.
Right arrow Articles by Nath, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Prakash, P.
Right arrow Articles by Nath, G.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, January 2005, p. 431-432, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.431-432.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Evaluation of Nested PCR in Diagnosis of Typhoid Fever

Pradyot Prakash,1* Om Prakash Mishra,2 Alok Kumar Singh,3 Anil Kumar Gulati,1 and Gopal Nath1

Department of Microbiology,1 Pediatrics, Institute of Medical Sciences,2 Department of Botany, Faculty of Science, Banaras Hindu University, Varanasi, India3

Received 24 February 2004/ Returned for modification 29 April 2004/ Accepted 3 September 2004

In this study, nested PCR using H1-d primers, which is specific for Salmonella enterica serovar Typhi, was compared to blood culture and the single-tube Widal test. Results indicate that nested PCR can be used as a gold standard to determine the cutoff titer of the Widal test for diagnosis of typhoid fever.

* Corresponding author. Mailing address: Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India. Phone: 91-9415201684. E-mail: pradyot_micro{at}yahoo.co.in.

Journal of Clinical Microbiology, January 2005, p. 431-432, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.431-432.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ou, H.-Y., Ju, C. T. S., Thong, K.-L., Ahmad, N., Deng, Z., Barer, M. R., Rajakumar, K. (2007). Translational Genomics to Develop a Salmonella enterica Serovar Paratyphi A Multiplex Polymerase Chain Reaction Assay. J. Mol. Diagn. 9: 624-630 [Abstract] [Full Text]  
  • HATTA, M., SMITS, H. L. (2007). DETECTION OF SALMONELLA TYPHI BY NESTED POLYMERASE CHAIN REACTION IN BLOOD, URINE, AND STOOL SAMPLES. Am J Trop Med Hyg 76: 139-143 [Abstract] [Full Text]  
  • Baker, S., Sarwar, Y., Aziz, H., Haque, A., Ali, A., Dougan, G., Wain, J., Haque, A. (2005). Detection of Vi-Negative Salmonella enterica Serovar Typhi in the Peripheral Blood of Patients with Typhoid Fever in the Faisalabad Region of Pakistan. J. Clin. Microbiol. 43: 4418-4425 [Abstract] [Full Text]  
  • Zuniga, J., Lillo, L., Shin, J. J., Machavarapu, R. L., Quiroga, T., Goycoolea, M., Matsuhiro, B., Aron-Hott, L., Godfrey, H. P., Cabello, F. C. (2005). Salmonella enterica Serovar Typhi O:1,9,12 Polysaccharide-Protein Conjugate as a Diagnostic Tool for Typhoid Fever. J. Clin. Microbiol. 43: 4545-4550 [Abstract] [Full Text]  
  • Arya, S. C., Agarwal, N., Prakash, P., Gulati, A. K., Nath, G. (2005). Nested PCR for Diagnosis of Typhoid Fever. J. Clin. Microbiol. 43: 4920-4920 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»