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Journal of Clinical Microbiology, January 2005, p. 431-432, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.431-432.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Evaluation of Nested PCR in Diagnosis of Typhoid Fever

Pradyot Prakash,1* Om Prakash Mishra,2 Alok Kumar Singh,3 Anil Kumar Gulati,1 and Gopal Nath1

Department of Microbiology,1 Pediatrics, Institute of Medical Sciences,2 Department of Botany, Faculty of Science, Banaras Hindu University, Varanasi, India3

Received 24 February 2004/ Returned for modification 29 April 2004/ Accepted 3 September 2004

In this study, nested PCR using H1-d primers, which is specific for Salmonella enterica serovar Typhi, was compared to blood culture and the single-tube Widal test. Results indicate that nested PCR can be used as a gold standard to determine the cutoff titer of the Widal test for diagnosis of typhoid fever.

* Corresponding author. Mailing address: Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India. Phone: 91-9415201684. E-mail: pradyot_micro{at}yahoo.co.in.

Journal of Clinical Microbiology, January 2005, p. 431-432, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.431-432.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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