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Journal of Clinical Microbiology, January 2005, p. 433-435, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.433-435.2005

Effect of Inoculum Size on Detection of Candida Growth by the BACTEC 9240 Automated Blood Culture System Using Aerobic and Anaerobic Media

Benjamin J. George, Lynn L. Horvath,* and Duane R. Hospenthal

Department of Medicine, Brooke Army Medical Center, Fort Sam Houston, Texas

Received 23 June 2004/ Returned for modification 4 August 2004/ Accepted 28 August 2004

Simulated candidemia was produced with 20 Candida isolates at three inoculum sizes (100, 10, and 1 CFU/ml of blood). Growth detection was better with larger inocula. The time to growth detection was shorter with larger inocula. Inoculum size does effect Candida growth detection and time to detection in BACTEC 9240 automated systems.

* Corresponding author. Mailing address: Brooke Army Medical Center, Infectious Disease Service (MCHE-MDI), 3851 Roger Brooke Dr., Fort Sam Houston, TX 78234. Phone: (210) 916-4355. Fax: (210) 916-0388. E-mail: Lynn.Horvath{at}amedd.army.mil.

Journal of Clinical Microbiology, January 2005, p. 433-435, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.433-435.2005




This article has been cited by other articles:

  • Horvath, L. L., George, B. J., Hospenthal, D. R. (2007). Detection of Fifteen Species of Candida in an Automated Blood Culture System. J. Clin. Microbiol. 45: 3062-3064 [Abstract] [Full Text]  


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