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Journal of Clinical Microbiology, January 2005, p. 436-438, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.436-438.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Evidence for Preferential Genotyping of a Minority Human Immunodeficiency Virus Population Due to Primer-Template Mismatching during PCR-Based Amplification

Javier Martinez-Picado,1* Kristina Morales-Lopetegi,1 Cristina Villena,1 Carolina Gutiérrez,2 Nuria Izquierdo,1 Silvia Marfil,1 Bonaventura Clotet,1 and Lidia Ruiz1

IrsiCaixa Foundation, Hospital Germans Trias i Pujol, Universitat Autònoma de Barcelona, Badalona,1 Hospital Ramón y Cajal, Madrid, Spain2

Received 2 April 2004/ Returned for modification 1 June 2004/ Accepted 31 August 2004

Human immunodeficiency virus type 1 (HIV-1) genotyping assays have come to be widely used for monitoring antiretroviral drug resistance. We report a case in which primer-template mismatches during nested PCR-based amplification biased the composition of the original viral population in the sample, magnifying a distinct minority HIV-1 population. This observation might help to explain some unexpected HIV-1 genotypes.

* Corresponding author. Mailing address: IrsiCaixa Foundation, Hospital Germans Trias i Pujol, Ctra. de Canyet s/n, 08916 Badalona, Spain. Phone: 34 93 4656374. Fax:. 34 93 4653968. E-mail: javiermp{at}ns.hugtip.scs.es.

Journal of Clinical Microbiology, January 2005, p. 436-438, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.436-438.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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Copyright © 2005 by the American Society for Microbiology. All rights reserved.