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Journal of Clinical Microbiology, January 2005, p. 436-438, Vol. 43, No. 1
0095-1137/05/$08.00+0 doi:10.1128/JCM.43.1.436-438.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
IrsiCaixa Foundation, Hospital Germans Trias i Pujol, Universitat Autònoma de Barcelona, Badalona,1 Hospital Ramón y Cajal, Madrid, Spain2
Received 2 April 2004/ Returned for modification 1 June 2004/ Accepted 31 August 2004
Human immunodeficiency virus type 1 (HIV-1) genotyping assays have come to be widely used for monitoring antiretroviral drug resistance. We report a case in which primer-template mismatches during nested PCR-based amplification biased the composition of the original viral population in the sample, magnifying a distinct minority HIV-1 population. This observation might help to explain some unexpected HIV-1 genotypes.
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