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Journal of Clinical Microbiology, January 2005, p. 436-438, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.436-438.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Evidence for Preferential Genotyping of a Minority Human Immunodeficiency Virus Population Due to Primer-Template Mismatching during PCR-Based Amplification

Javier Martinez-Picado,1* Kristina Morales-Lopetegi,1 Cristina Villena,1 Carolina Gutiérrez,2 Nuria Izquierdo,1 Silvia Marfil,1 Bonaventura Clotet,1 and Lidia Ruiz1

IrsiCaixa Foundation, Hospital Germans Trias i Pujol, Universitat Autònoma de Barcelona, Badalona,1 Hospital Ramón y Cajal, Madrid, Spain2

Received 2 April 2004/ Returned for modification 1 June 2004/ Accepted 31 August 2004

Human immunodeficiency virus type 1 (HIV-1) genotyping assays have come to be widely used for monitoring antiretroviral drug resistance. We report a case in which primer-template mismatches during nested PCR-based amplification biased the composition of the original viral population in the sample, magnifying a distinct minority HIV-1 population. This observation might help to explain some unexpected HIV-1 genotypes.

* Corresponding author. Mailing address: IrsiCaixa Foundation, Hospital Germans Trias i Pujol, Ctra. de Canyet s/n, 08916 Badalona, Spain. Phone: 34 93 4656374. Fax:. 34 93 4653968. E-mail: javiermp{at}ns.hugtip.scs.es.

Journal of Clinical Microbiology, January 2005, p. 436-438, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.436-438.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Parisi, S. G., Boldrin, C., Cruciani, M., Nicolini, G., Cerbaro, I., Manfrin, V., Dal Bello, F., Franchin, E., Franzetti, M., Rossi, M. C., Cattelan, A. M., Romano, L., Zazzi, M., Andreoni, M., Palu, G. (2007). Both Human Immunodeficiency Virus Cellular DNA Sequencing and Plasma RNA Sequencing Are Useful for Detection of Drug Resistance Mutations in Blood Samples from Antiretroviral-Drug-Naive Patients. J. Clin. Microbiol. 45: 1783-1788 [Abstract] [Full Text]  


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