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Journal of Clinical Microbiology, January 2005, p. 442-444, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.442-444.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Cetyl-Pyridinium Chloride Is Useful for Isolation of Mycobacterium tuberculosis from Sputa Subjected to Long-Term Storage

Manuela Pardini,1 Francis Varaine,2 Elisabetta Iona,1 Erchanik Arzumanian,3 Francesco Checchi,4 Marco Rinaldo Oggioni,5 Graziella Orefici,1 and Lanfranco Fattorini1*

Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanità, Rome,1 Dipartimento di Biologia Molecolare, Università di Siena, Siena, Italy,5 Médecins Sans Frontières,2 Epicentre, Paris, France,4 Guliripchi Tuberculosis Hospital, Sukhumi, Abkhazia3

Received 27 May 2004/ Returned for modification 6 July 2004/ Accepted 31 August 2004

Recovery of Mycobacterium tuberculosis from sputa treated with cetyl-pyridinium chloride (CPC) and stored for 20 ± 9 days was significantly higher than that from sputa that were untreated and processed by the N-acetyl-L-cisteine-NaOH method. Addition of CPC is useful for isolation of M. tuberculosis from sputa subjected to long-term storage received from remote areas of the world.

* Corresponding author. Mailing address: Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy. Phone: 39 06 49903167. Fax: 39 06 49387112. E-mail: fattolan{at}iss.it.

Journal of Clinical Microbiology, January 2005, p. 442-444, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.442-444.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Pardini, M., Varaine, F., Bonnet, M., Orefici, G., Oggioni, M. R., the LONG-DRUG Study Group, , Fattorini, L. (2007). Usefulness of the BACTEC MGIT 960 System for Isolation of Mycobacterium tuberculosis from Sputa Subjected to Long-Term Storage. J. Clin. Microbiol. 45: 575-576 [Abstract] [Full Text]  


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