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Journal of Clinical Microbiology, January 2005, p. 442-444, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.442-444.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Cetyl-Pyridinium Chloride Is Useful for Isolation of Mycobacterium tuberculosis from Sputa Subjected to Long-Term Storage

Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanità, Rome,¹ Dipartimento di Biologia Molecolare, Università di Siena, Siena, Italy,⁵ Médecins Sans Frontières,² Epicentre, Paris, France,⁴ Guliripchi Tuberculosis Hospital, Sukhumi, Abkhazia³

Received 27 May 2004/ Returned for modification 6 July 2004/ Accepted 31 August 2004

Recovery of Mycobacterium tuberculosis from sputa treated with cetyl-pyridinium chloride (CPC) and stored for 20 ± 9 days was significantly higher than that from sputa that were untreated and processed by the N-acetyl-L-cisteine-NaOH method. Addition of CPC is useful for isolation of M. tuberculosis from sputa subjected to long-term storage received from remote areas of the world.

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