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Journal of Clinical Microbiology, January 2005, p. 491-493, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.491-493.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Variable gp43 Secretion by Paracoccidioides brasiliensis Clones Obtained by Two Different Culture Methods

Rodrigo Berzaghi, Silvia Helena Marques da Silva, and Zoilo Pires de Camargo*

Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo, São Paulo, Brazil

Received 13 January 2004/ Returned for modification 20 February 2004/ Accepted 7 July 2004

The main objectives of this study were to obtain clones of Paracoccidioides brasiliensis by two methods (micromanipulation and plating assay) and to determine if the secretion of the 43-kDa glycoprotein (gp43) is dependent on the clonal culture. The results show that the secretion of gp43 is not dependent on clonal cultures. Clones that originally were secretors of this molecule, after subculturing, lost this characteristic; on the other hand, clones that originally did not secrete gp43 began to secrete gp43 after subculturing.

* Corresponding author. Mailing address: Universidade Federal de São Paulo, Disciplina de Biologia Celular, 04023-062, Rua Botucatu, 862, 8° andar, São Paulo, SP., Brazil. Phone: 55 11 5576 45 23. Fax: 55 11 5571 58 77. E-mail: zoilo{at}ecb.epm.br.

Journal of Clinical Microbiology, January 2005, p. 491-493, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.491-493.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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