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Journal of Clinical Microbiology, January 2005, p. 509-510, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.509-510.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Comparison of the Mycoplasma Duo Test with PCR for Detection of Ureaplasma Species in Endotracheal Aspirates from Premature Infants

Fook-Choe Cheah,1,2,{dagger} Trevor P. Anderson,3 Brian A. Darlow,2 and David R. Murdoch1,3*

Departments of Pathology,1 Paediatrics, Christchurch School of Medicine and Health Sciences, University of Otago,2 Microbiology Unit, Canterbury Health Laboratories, Christchurch, New Zealand3

Received 7 June 2004/ Returned for modification 28 July 2004/ Accepted 17 September 2004

We compared the Mycoplasma Duo kit (Sanofi Diagnostics Pasteur) with PCR for detection of Ureaplasma spp. in endotracheal aspirates from 60 premature neonates. The overall agreement between the two tests was 96%. The Mycoplasma Duo assay is a useful alternative to culture and PCR for detection of neonatal Ureaplasma infection.

* Corresponding author. Mailing address: Department of Pathology, Christchurch School of Medicine and Health Sciences, P.O. Box 4345, Christchurch, New Zealand. Phone: 64-3-364 0587. Fax: 64-3-364 0009. E-mail: david.murdoch{at}cdhb.govt.nz.

{dagger} Present address: Department of Paediatrics, Faculty of Medicine, Universiti Kebangsaan Malaysia, Bandar Tun Razak, 56000 Kuala Lumpur, Malaysia.

Journal of Clinical Microbiology, January 2005, p. 509-510, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.509-510.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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