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Journal of Clinical Microbiology, January 2005, p. 84-88, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.84-88.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Performance of the New VITEK 2 GP Card for Identification of Medically Relevant Gram-Positive Cocci in a Routine Clinical Laboratory

Guido Funke* and Pascale Funke-Kissling

Department of Medical Microbiology and Hygiene, Gärtner and Colleagues Laboratories, Weingarten, Germany

Received 11 May 2004/ Returned for modification 18 June 2004/ Accepted 4 August 2004

The VITEK 2 gram-positive (GP) identification card (bioMérieux, Marcy l'Etoile, France) has been redesigned to achieve greater accuracy in the identification of gram-positive cocci. A total of 43 biochemical tests, including 17 enzymatic tests, are present in the card and interpreted in a kinetic mode, for up to 8 h. The VITEK 2 database, used in conjunction with the GP identification card, allows the identification of 115 different taxa. A total of 364 strains of GP cocci (217 Streptococcaceae strains and 147 Micrococcaceae strains) belonging to 31 taxa were tested with the new VITEK 2 GP identification card. Of the 364 strains, 105 were taken from routine primary plating media. A total of 344 strains (94.5%) were correctly identified to the species level and 17 strains (4.7%) were identified with low discrimination, requiring additional tests, whereas 1 strain (0.3%) was incorrectly identified and 2 strains (0.5%) remained unidentified. Within 7 h of the start of incubation, more than 90% of all strains were identified. Of the 105 primary cultures, 97% were correctly identified to the species level, 2% were identified with low discrimination, and 1% remained unidentified. Identification performance data were independent of each of the three plating media used. It is concluded that the new VITEK 2 GP identification card provides reliable results for the identification of GP cocci under routine laboratory conditions.

* Corresponding author. Mailing address: Department of Medical Microbiology and Hygiene, Gärtner and Colleagues Laboratories, Hoyerstrasse 51, D-88250 Weingarten, Germany. Phone: 49-751-502-630. Fax: 49-751-502-385. E-mail: ldg.funke{at}t-online.de.

Journal of Clinical Microbiology, January 2005, p. 84-88, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.84-88.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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