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Journal of Clinical Microbiology, October 2005, p. 5278-5280, Vol. 43, No. 10
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.10.5278-5280.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of the DiversiLab System for Species and Strain Differentiation of Fusarium Species Isolates

M. Healy,1* K. Reece,1 D. Walton,1 J. Huong,1 S. Frye,1 I. I. Raad,2 and D. P. Kontoyiannis2*

Bacterial Barcodes, Inc.,1 Department of Infectious Diseases, Infection Control and Employee Health, The University of Texas M. D. Anderson Cancer Center, Houston, Texas2

Received 13 April 2005/ Returned for modification 31 May 2005/ Accepted 12 July 2005

Advances in molecular typing of fusariosis would facilitate the study of its epidemiology. We tested 26 such isolates by the commercially available DiversiLab System. The system utilizes automated repetitive sequence-based PCR (rep-PCR) and web-based data analyses. rep-PCR dendrogram cluster analysis showed agreement with species sequence identification (elongation factor 1 alpha gene). Additionally, subtype differences within the same species were noted.

* Corresponding author. Mailing address for D. P. Kontoyiannis: Department of Infectious Diseases, Infection Control and Employee Health, Unit 402, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030. Phone: (713) 792-6237. Fax: (713) 745-6839. E-mail: dkontoyi{at}mdanderson.org. Mailing address for M. Healy: Bacterial Barcodes, Inc., 8080 North Stadium Drive, Suite 1200, Houston, TX 77054. Phone: (713) 467-8500. Fax: (713) 467-7766. E-mail: mhealy{at}bacbarcodes.com.

Journal of Clinical Microbiology, October 2005, p. 5278-5280, Vol. 43, No. 10
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.10.5278-5280.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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