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Journal of Clinical Microbiology, October 2005, p. 5281-5284, Vol. 43, No. 10
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.10.5281-5284.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Comparative Nucleotide Sequence Analysis of Polymorphic Variable-Number Tandem-Repeat Loci in Mycobacterium ulcerans

Anthony Ablordey,1 Markus Hilty,2 Pieter Stragier,1 Jean Swings,3,4 and Françoise Portaels1*

Mycobacteriology Unit, Institute of Tropical Medicine, B-2000 Antwerp, Belgium,1 Swiss Tropical Institute, 4002, Basel, Switzerland,2 Laboratory of Microbiology,3 BCCM/LMG Culture Collection, University Ghent, B-9000 Ghent, Belgium4

Received 19 April 2005/ Returned for modification 1 July 2005/ Accepted 7 July 2005

We analyzed a set of variable-number tandem-repeat (VNTR) loci to assess their nucleotide sequence diversity in isolates of three Mycobacterium ulcerans genotypes. Sequence variants in two loci resulted in intraspecies resolution of Southeast Asian and Asian genotypes in contrast to a homogenous sequence composition among African isolates. Nucleotide sequence polymorphism in repeat units can enhance discrimination of VNTR loci.

* Corresponding author. Mailing address: Mycobacteriology Unit, Prince Leopold Institute of Tropical Medicine, B-2000 Antwerp, Belgium. Phone: 32-3-247-63-17. Fax: 32-3-247-63-33. E-mail: portaels{at}itg.be.

Journal of Clinical Microbiology, October 2005, p. 5281-5284, Vol. 43, No. 10
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.10.5281-5284.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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