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Journal of Clinical Microbiology, October 2005, p. 5309-5311, Vol. 43, No. 10
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.10.5309-5311.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Comparison of API 50 CH Strips to Whole-Chromosomal DNA Probes for Identification of Lactobacillus Species

Melinda A. Boyd,1 May A. D. Antonio,1 and Sharon L. Hillier1,2*

Magee-Womens Research Institute,1 Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania2

Received 19 April 2005/ Returned for modification 8 June 2005/ Accepted 30 June 2005

The API 50 CH identification system was evaluated for the identification of 97 strains of commensal lactobacilli. This system agreed with the species-level identifications for none of the 7 reference strains and only 4 of 90 vaginal isolates identified using whole-chromosomal DNA probes.

* Corresponding author. Mailing address: University of Pittsburgh, Department of Obstetrics, Gynecology, and Reproductive Sciences, Magee-Womens Hospital, 300 Halket Street, Pittsburgh, PA 15213-3180. Phone: (412) 641-6416. Fax: (412) 641-1133. E-mail: slh6+{at}pitt.edu.

Journal of Clinical Microbiology, October 2005, p. 5309-5311, Vol. 43, No. 10
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.10.5309-5311.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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