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Journal of Clinical Microbiology, October 2005, p. 5327-5331, Vol. 43, No. 10
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.10.5327-5331.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Sequence-Based Typing of adeB as a Potential Tool To Identify Intraspecific Groups among Clinical Strains of Multidrug-Resistant Acinetobacter baumannii

Laboratory of Microbiology,¹ BCCM/LMG Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, Ghent, Belgium,⁴ National Institute of Public Health,² Department of Medical Microbiology, 3rd Faculty of Medicine, Charles University, Prague, Czech Republic³

Received 20 May 2005/ Returned for modification 20 June 2005/ Accepted 22 July 2005

Sequence analysis of an 850-bp fragment internal to the aspecific drug efflux gene adeB revealed 11 sequence types (STs) among a collection of 50 multidrug-resistant Acinetobacter baumannii (MDRAB) strains, including members of pan-European clones I, II, and III. The delineation of STs conformed with the intraspecific grouping of these strains previously determined by different DNA fingerprinting methods. Larger strain collections need to be screened to further explore the potential of sequence-based adeB typing as a universal tool for the monitoring of MDRAB clones.

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