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Journal of Clinical Microbiology, October 2005, p. 5341-5343, Vol. 43, No. 10
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.10.5341-5343.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of a Selective Enrichment Broth To Recover Clostridium difficile from Stool Swabs Stored under Different Conditions

Luis G. Arroyo,1* Joyce Rousseau,1 Barbara M. Willey,2 Don E. Low,2 Henry Staempfli,1 Allison McGeer,2 and J. Scott Weese1

Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada,1 Department of Microbiology, Mount Sinai Hospital, Toronto, Ontario, Canada2

Received 17 June 2005/ Accepted 25 July 2005

The recovery of Clostridium difficile from the stools of patients with C. difficile-associated diarrhea was evaluated by use of an enrichment broth (cycloserine-cefoxitin fructose broth supplemented with 0.1% sodium taurocholate [TCCFB]) and was compared to that from selective agar (cycloserine-cefoxitin fructose agar [CCFA]) and alcohol shock followed by inoculation onto blood agar (AS-BA). TCCFB was superior to CCFA and AS-BA, and neither the storage time nor the storage temperature affected the recovery rate.

* Corresponding author. Mailing address: Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, Canada. Phone: (519) 823-8840. Fax: (519) 763-9544. E-mail: larroyo{at}uoguelph.ca.

Journal of Clinical Microbiology, October 2005, p. 5341-5343, Vol. 43, No. 10
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.10.5341-5343.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Martin, H., Willey, B., Low, D. E., Staempfli, H. R., McGeer, A., Boerlin, P., Mulvey, M., Weese, J. S. (2008). Characterization of Clostridium difficile Strains Isolated from Patients in Ontario, Canada, from 2004 to 2006. J. Clin. Microbiol. 46: 2999-3004 [Abstract] [Full Text]  
  • Martirosian, G. (2006). Recovery of Clostridium difficile from Hospital Environments.. J. Clin. Microbiol. 44: 1202-1203 [Full Text]  


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