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Journal of Clinical Microbiology, October 2005, p. 5348-5350, Vol. 43, No. 10
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.10.5348-4350.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Comparison of Three Molecular Methods Used for Subtyping of Legionella pneumophila Strains Isolated during an Epidemic of Legionellosis in Rome

M. Scaturro,* M. Losardo, G. De Ponte, and M. L. Ricci

Department of Infectious Parasitic and Immunomediate Diseases, Istituto Superiore di Sanità, Rome, Italy

Received 24 May 2005/ Returned for modification 8 July 2005/ Accepted 26 July 2005

In the summer of 2003 a community-acquired outbreak of Legionella pneumophila occurred in Rome, Italy. Three molecular typing methods, pulse-field gel electrophoresis, amplified fragment length polymorphism analysis, and sequence-based typing (SBT), were used to establish the clonal correlation among the isolates of the epidemic cluster. By comparison of the methods, SBT was the most rapid and the easiest to perform and provided unambiguous results.

* Corresponding author. Mailing address: Department of Infectious Parasitic and Immunomediate Diseases, Istituto Superiore di Sanità, Viale Regina Elena, 299-00161 Rome, Italy. Phone: 390649902856. Fax: 390649387112. E-mail: marisa.scaturro{at}iss.it.

Journal of Clinical Microbiology, October 2005, p. 5348-5350, Vol. 43, No. 10
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.10.5348-4350.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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