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Journal of Clinical Microbiology, October 2005, p. 5359-5361, Vol. 43, No. 10
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.10.5359-5361.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Comparison of Ashdown's Medium, Burkholderia cepacia Medium, and Burkholderia pseudomallei Selective Agar for Clinical Isolation of Burkholderia pseudomallei

Sharon J. Peacock,1,2* Grace Chieng,1,3 Allen C. Cheng,1,4 David A. B. Dance,5 Premjit Amornchai,1 Gumphol Wongsuvan,1 Nittaya Teerawattanasook,6 Wirongrong Chierakul,1 Nicholas P. J. Day,1,2 and Vanaporn Wuthiekanun1

Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand,1 Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, Churchill Hospital, Oxford OX3 7LJ, United Kingdom,2 Faculty of Medicine, University of Melbourne, Melbourne, Australia,3 Menzies School of Health Research, Charles Darwin University and Northern Territory Clinical School, Flinders University, Darwin, Australia,4 Health Protection Agency, Tamar Science Park, Plymouth, Devon, United Kingdom,5 Medical Department, Sappasithiprasong Hospital, Ubon Ratchathani, Thailand6

Received 14 June 2005/ Returned for modification 1 July 2005/ Accepted 15 July 2005

Ashdown's medium, Burkholderia pseudomallei selective agar (BPSA), and a commercial Burkholderia cepacia medium were compared for their abilities to grow B. pseudomallei from 155 clinical specimens that proved positive for this organism. The sensitivity of each was equivalent; the selectivity of BPSA was lower than that of Ashdown's or B. cepacia medium.


* Corresponding author. Mailing address: Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Road, Bangkok 10400, Thailand. Phone: 66 2 354 9172. Fax: 66 2 354 9169. E-mail: sharon{at}tropmedres.ac.


Journal of Clinical Microbiology, October 2005, p. 5359-5361, Vol. 43, No. 10
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.10.5359-5361.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.




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