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Journal of Clinical Microbiology, October 2005, p. 5362-5365, Vol. 43, No. 10
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.10.5362-5365.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Single Multiplex PCR Assay To Identify Simultaneously the Six Categories of Diarrheagenic Escherichia coli Associated with Enteric Infections

Programa de Microbiología, ICBM, Facultad de Medicina, Universidad de Chile, Santiago, Chile,¹ Centro para Vacunas en Desarrollo, Santiago, Chile,² Center for Vaccine Development, University of Maryland, Baltimore, Maryland³

Received 30 March 2005/ Returned for modification 25 May 2005/ Accepted 12 July 2005

We designed a multiplex PCR for the detection of all categories of diarrheagenic Escherichia coli. This method proved to be specific and rapid in detecting virulence genes from Shiga toxin-producing (stx₁, stx₂, and eae), enteropathogenic (eae and bfp), enterotoxigenic (stII and lt), enteroinvasive (virF and ipaH), enteroaggregative (aafII), and diffuse adherent (daaE) Escherichia coli in stool samples.

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