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Journal of Clinical Microbiology, October 2005, p. 5369-5371, Vol. 43, No. 10
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.10.5369-5371.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of Specific rRNA Oligonucleotide Probes for Microscopic Detection of Mycobacterium tuberculosis in Culture and Tissue Specimens

Allison L. St. Amand,1 Daniel N. Frank,1 Mary Ann De Groote,1,2 Randall J. Basaraba,3 Ian M. Orme,3 and Norman R. Pace1*

Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347,1 Department of Medicine, Division of Infectious Diseases, University of Colorado Health Sciences Center, Denver, Colorado 80262,2 Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523-16193

Received 5 May 2005/ Returned for modification 20 July 2005/ Accepted 31 July 2005

Mycobacterium tuberculosis infections are a major global health problem. Fast and accurate detection of M. tuberculosis is clearly needed at both the clinical level and the research level. We report a rapid and reliable in situ hybridization technique using rRNA oligonucleotide probes for the identification of M. tuberculosis in tissues and cultures.

* Corresponding author. Mailing address: Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309-0347. Phone: (303) 735-1864. Fax: (303) 492-7744. E-mail: nrpace{at}colorado.edu.

Journal of Clinical Microbiology, October 2005, p. 5369-5371, Vol. 43, No. 10
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.10.5369-5371.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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