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Journal of Clinical Microbiology, November 2005, p. 5470-5476, Vol. 43, No. 11
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.11.5470-5476.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Cultivable Oral Microbiota of Domestic Dogs

David R. Elliott,1 Michael Wilson,1 Catherine M. F. Buckley,2 and David A. Spratt1*

Microbiology Unit, Division of Microbial Diseases, Eastman Dental Institute, UCL, 256 Gray's Inn Road, London WC1X 8LD, United Kingdom,1 Waltham Centre for Pet Nutrition, Waltham-on-the-Wolds, Melton Mowbray, Leicestershire LE14 4RT, United Kingdom2

Received 23 February 2005/ Returned for modification 13 May 2005/ Accepted 24 July 2005

Bacteria were isolated from the dental plaques of nine dogs and a sample of pooled saliva from five other dogs and were then identified by comparative 16S rRNA gene sequencing. Among 339 isolates, 84 different phylotypes belonging to 37 genera were identified. Approximately half of the phylotypes were identified to the species level, and 28% of these were considered members of the indigenous oral microbiota of humans. The 16S rRNA gene sequences of the remaining 44 phylotypes were not represented in GenBank, and most of these phylotypes were tentatively identified as candidate new species. The genera most frequently isolated from saliva were Actinomyces (26%), Streptococcus (18%), and Granulicatella (17%). The genera most frequently isolated from plaque were Porphyromonas (20%), Actinomyces (12%), and Neisseria (10%). A comparison of the DNA sequences from this study with sequences of the human microbiota available in GenBank showed that, on average, canine and human microbiotas differed by almost 7% in the 16S rRNA gene. In conclusion, this study has shown that the cultivable oral microbiotas of dogs and humans show significant differences.

* Corresponding author. Mailing address: Microbiology Unit, Division of Microbial Diseases, Eastman Dental Institute for Oral Health Care Sciences, University College London, 256 Gray's Inn Road, London WC1X 8LD, United Kingdom. Phone: 44 20 7915 1107. Fax: 44 20 7915 1127. E-mail: d.spratt{at}eastman.ucl.ac.uk.

Journal of Clinical Microbiology, November 2005, p. 5470-5476, Vol. 43, No. 11
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.11.5470-5476.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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